tslp elisa development kit Search Results


tslp elisa kit  (R&D Systems)
94
R&D Systems tslp elisa kit
Figure 2 <t>TSLP</t> and TSLPR expression in DCs of nasally immunized mice. Mice were nasally immunized weekly for 3 consecutive weeks with PspA only, CT only, or PspA plus CT. All the samples were harvested at 1 week after the last immunization. (a) RT-qPCR analysis of TSLP mRNA expression in CD11c þ DCs from spleen, CLN, NALT, NP, and lung tissue of nasally immunized mice. CD11c þ DCs were isolated by MACS and pooled from three mice per group. The mRNA expression level was normalized to that of b actin, and data are expressed relative to the value in PspA only DCs. Values represent the mean±s.e.m. of values from three independent experiments. **Po0.01 vs. PspA only DCs. (b) Flow cytometric analysis of TSLPR expression in CD11b þCD11c þ DCs from spleen, NP, lung tissue of nasally immunized mice (open histogram). Shaded histograms indicate isotype control. MFI indicating TSLPR expression is shown. Values are means±s.e.m. (n ¼ 3 per group). Data are representative of three independent experiments. *Po0.05 vs. mice nasally immunized with PspA plus CT. CLN, cervical lymph node; CT, cholera toxin; DC, dendritic cells; MACS, magnetic-activated cell sorting; MFI, mean fluorescence intensity; mRNA,messenger RNA; NP,nasal passages; NS, not significant; PspA, pneumococcal surface protein A; RT- qPCR, real-time-quantitative PCR; TSLP, thymic stromal lymphopoietin.
Tslp Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tslp elisa kit/product/R&D Systems
Average 94 stars, based on 1 article reviews
tslp elisa kit - by Bioz Stars, 2026-05
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tslp elisa development kit  (PeproTech)
90
PeproTech tslp elisa development kit
Figure 2 <t>TSLP</t> and TSLPR expression in DCs of nasally immunized mice. Mice were nasally immunized weekly for 3 consecutive weeks with PspA only, CT only, or PspA plus CT. All the samples were harvested at 1 week after the last immunization. (a) RT-qPCR analysis of TSLP mRNA expression in CD11c þ DCs from spleen, CLN, NALT, NP, and lung tissue of nasally immunized mice. CD11c þ DCs were isolated by MACS and pooled from three mice per group. The mRNA expression level was normalized to that of b actin, and data are expressed relative to the value in PspA only DCs. Values represent the mean±s.e.m. of values from three independent experiments. **Po0.01 vs. PspA only DCs. (b) Flow cytometric analysis of TSLPR expression in CD11b þCD11c þ DCs from spleen, NP, lung tissue of nasally immunized mice (open histogram). Shaded histograms indicate isotype control. MFI indicating TSLPR expression is shown. Values are means±s.e.m. (n ¼ 3 per group). Data are representative of three independent experiments. *Po0.05 vs. mice nasally immunized with PspA plus CT. CLN, cervical lymph node; CT, cholera toxin; DC, dendritic cells; MACS, magnetic-activated cell sorting; MFI, mean fluorescence intensity; mRNA,messenger RNA; NP,nasal passages; NS, not significant; PspA, pneumococcal surface protein A; RT- qPCR, real-time-quantitative PCR; TSLP, thymic stromal lymphopoietin.
Tslp Elisa Development Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tslp elisa development kit/product/PeproTech
Average 90 stars, based on 1 article reviews
tslp elisa development kit - by Bioz Stars, 2026-05
90/100 stars
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tslp elisa development kit  (Novus Biologicals)
N/A
TSLP ELISA Development Kit
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Figure 2 TSLP and TSLPR expression in DCs of nasally immunized mice. Mice were nasally immunized weekly for 3 consecutive weeks with PspA only, CT only, or PspA plus CT. All the samples were harvested at 1 week after the last immunization. (a) RT-qPCR analysis of TSLP mRNA expression in CD11c þ DCs from spleen, CLN, NALT, NP, and lung tissue of nasally immunized mice. CD11c þ DCs were isolated by MACS and pooled from three mice per group. The mRNA expression level was normalized to that of b actin, and data are expressed relative to the value in PspA only DCs. Values represent the mean±s.e.m. of values from three independent experiments. **Po0.01 vs. PspA only DCs. (b) Flow cytometric analysis of TSLPR expression in CD11b þCD11c þ DCs from spleen, NP, lung tissue of nasally immunized mice (open histogram). Shaded histograms indicate isotype control. MFI indicating TSLPR expression is shown. Values are means±s.e.m. (n ¼ 3 per group). Data are representative of three independent experiments. *Po0.05 vs. mice nasally immunized with PspA plus CT. CLN, cervical lymph node; CT, cholera toxin; DC, dendritic cells; MACS, magnetic-activated cell sorting; MFI, mean fluorescence intensity; mRNA,messenger RNA; NP,nasal passages; NS, not significant; PspA, pneumococcal surface protein A; RT- qPCR, real-time-quantitative PCR; TSLP, thymic stromal lymphopoietin.

Journal: Mucosal immunology

Article Title: Critical role of TSLP-responsive mucosal dendritic cells in the induction of nasal antigen-specific IgA response.

doi: 10.1038/mi.2016.103

Figure Lengend Snippet: Figure 2 TSLP and TSLPR expression in DCs of nasally immunized mice. Mice were nasally immunized weekly for 3 consecutive weeks with PspA only, CT only, or PspA plus CT. All the samples were harvested at 1 week after the last immunization. (a) RT-qPCR analysis of TSLP mRNA expression in CD11c þ DCs from spleen, CLN, NALT, NP, and lung tissue of nasally immunized mice. CD11c þ DCs were isolated by MACS and pooled from three mice per group. The mRNA expression level was normalized to that of b actin, and data are expressed relative to the value in PspA only DCs. Values represent the mean±s.e.m. of values from three independent experiments. **Po0.01 vs. PspA only DCs. (b) Flow cytometric analysis of TSLPR expression in CD11b þCD11c þ DCs from spleen, NP, lung tissue of nasally immunized mice (open histogram). Shaded histograms indicate isotype control. MFI indicating TSLPR expression is shown. Values are means±s.e.m. (n ¼ 3 per group). Data are representative of three independent experiments. *Po0.05 vs. mice nasally immunized with PspA plus CT. CLN, cervical lymph node; CT, cholera toxin; DC, dendritic cells; MACS, magnetic-activated cell sorting; MFI, mean fluorescence intensity; mRNA,messenger RNA; NP,nasal passages; NS, not significant; PspA, pneumococcal surface protein A; RT- qPCR, real-time-quantitative PCR; TSLP, thymic stromal lymphopoietin.

Article Snippet: The concentration of TSLP in broncho-alveolar fluid samples was measured by using a TSLP ELISA kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Quantitative RT-PCR, Isolation, Control, FACS, Fluorescence, Real-time Polymerase Chain Reaction